Pcr as a diagnostic tool for brucellosis

Some features of the site may not work correctly. DOI: Bricker Published 20 December Biology, Medicine Veterinary microbiology Numerous PCR-based assays have been developed for the identification of Brucella to improve diagnostic capabilities. Collectively, the repertoire of assays addresses several aspects of the diagnostic process. For some purposes, the simple identification of Brucella is adequate e. In these cases, a genus-specific PCR assay is sufficient. Genus-specific assays tend to be simple, robust, and somewhat permissive of environmental… Expand.

View on PubMed. Save to Library Save. Create Alert Alert. Share This Paper. Background Citations. Methods Citations. Results Citations.

Topics from this paper. Citation Type. Has PDF. Publication Type. More Filters. Evaluation of genus-specific and species-specific real-time PCR assays for the identification of Brucella spp. Clinical chemistry and laboratory medicine. Diagnosis of human brucellosis using PCR. Expert review of molecular diagnostics.

Real-time PCR for identification of Brucella spp. Veterinary microbiology. View 2 excerpts, cites methods. View 1 excerpt, cites methods.

Polymerase chain reaction—based assays for the diagnosis of human brucellosis. Annals of Clinical Microbiology and Antimicrobials. For example, most government-sponsored brucellosis eradication programs include regulations that stipulate a species-specific response.

For epidemiological trace back, strain-specific identification is helpful. Typically, differential PCR-based assays tend to be more complex and consequently more difficult to perform. Several strategies have been explored to differentiate among Brucella species and strains, including locus specific multiplexing e.

This paper reviews some of the major advancements in molecular diagnostics for Brucella including the development of procedures designed for the direct analysis of a variety of clinical samples. While the progress to date is impressive, there is still room for improvement. Abstract Numerous PCR-based assays have been developed for the identification of Brucella to improve diagnostic capabilities.